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KMID : 1007520110200061609
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Food Science and Biotechnology
2011 Volume.20 No. 6 p.1609 ~ p.1614
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Alternative Extraction Technique for the Quantitative Analysis of Succinic Acid in a Chitosan-succinic Acid Powder Mixture Using GC-MS
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Lee Do-Gyeong
Park Ji-Hae
Yun Jee-Hye
Song Myung-Chong
Ahn Eun-Mi
Chung In-Sik
Baek Nam-In
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Abstract
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Typical solvent extraction of succinic acid from a chitosan-succinic acid powder mixture gave unsatisfactory yield (10.0%) due to the presence of intramolecular crosslinks between the chitosan amine groups and succinic acid carboxylic groups. An improved extraction method, which included hydrolysis of cross-links by KOH to liberate succinic acid from the matrix, afforded satisfactory yield (95.2±4.0%). Quantitative analysis of succinic acid in chitosan-succinic acid mixture was performed using GCMS and the optimized analysis method showed good validation in terms of specificity, linearity, accuracy (recovery rate), precision (inter-day and intra-day precision), limit of quantification (LOQ), and limit of detection (LOD).
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KEYWORD
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alkaline hydrolysis, chitosan, GC-MS, succinic acid, validation
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